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BIOSAFETY CABINET
WORK PRACTICES
- Do not allow others to walk rapidly behind you when you are
working.
- Be aware of drafts from ventilation systems, doors, windows,
fans, etc.
- Plan procedures carefully.
- Start-up biosafety cabinet and decontaminate with Wescodyne
or suitable disinfectant.
- Let biosafety cabinet run at least 5 minutes to purge air
in the work area.
- Do not place items over front grille, do not block back grille.
- Separate clean and contaminated items.
- Use plastic backed towels on work surface to absorb spills.
- Minimize storage items in or near the biosafety cabinet.
- Wash hands and arms before and after work.
- Wear a rear tie gown with gathered/knitted cuffs.
- Wear long sleeve nitrile or latex gloves.
- Minimize movement of contaminated items over clean items
(work from clean to dirty).
- Perform work 6 inches back of front intake grille.
- Hold tubes, bottles, and other vessels as horizontal as possible.
- Use horizontal pipette discard pan with disinfectant such
as Wescodyne.
- Do not use vertical pipette canisters or use them outside
of a biosafety cabinet.
- Ultraviolet lights are not needed if good technique is followed.
- Do not overload cabinet. Nothing should pass out of cabinet
until work is complete.
- Place clean items toward front, contaminated toward rear.
- Remove contaminated items only after sealed or decontaminated.
- Do not use a flame. Turbulence and
filter damage may occur.
- Use disposable loops, swabs, needles, syringes, etc. whenever
possible.
- Do not use a biosafety cabinet for storage.
- Equipment that causes turbulence (centrifuge, blender, etc.)
should be placed in back 1/3 of work surface.
All other work in the cabinet should stop while apparatus is
running.
- Decontaminate after work is complete with Wescodyne or other
appropriate disinfectant.
Biosafety Cabinet UMB Policy
CDC NIH Biosafety
Cabinet Guideline
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